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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in many tumors, which results in chromosome instability, and so may possibly lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important position in marketing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are actually explained. Two supplemental splice variants had been described but specific nucleotide sequence data isn't nevertheless obtainable.
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Blocking peptides are peptides that bind specifically on the target antibody and block antibody binding. These peptide commonly consists of the epitope regarded from the antibody.
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This information outlines even further doable optimization of cellular and immunoassay parameters to make sure the absolute best final results are...
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This guideline outlines further more possible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope around the target protein. By comparing the staining from the blocked antibody versus the antibody by itself, you can see which staining is distinct.
Focus on Data Cyclin E belongs for the very conserved cyclin loved ones, whose members are characterised by a spectacular periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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